• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDCD2L Monoclonal antibody

PDCD2L Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G12C4

Cat no : 68249-1-Ig

Synonyms

PDCD2L, programmed cell death 2 like

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PDCD2L Monoclonal antibody (68249-1-Ig)

    WB analysis using 68249-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using PDCD2L Monoclonal antibody (68249-1-Ig)

    WB analysis using 68249-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PDCD2L Monoclonal antibody (68249-1-Ig)

    IF Staining of HEK-293 using 68249-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDCD2L antibody (68249-1-Ig, Clone: 2G12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HCT 116 cells, HeLa cells, K-562 cells, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68249-1-Ig targets PDCD2L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDCD2L fusion protein Ag30807 相同性解析による交差性が予測される生物種
Full Name programmed cell death 2-like
Calculated molecular weight 358 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC006146
Gene symbol PDCD2L
Gene ID (NCBI) 84306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDCD2L was aberrantly expressed in multiple types of human cancers, and associated with clinical stage and molecular subtype and PDCD2L could be served as a biomarker of CRC (PMID: 35216602).

Protocols

Product Specific Protocols
WB protocol for PDCD2L antibody 68249-1-IgDownload protocol
IF protocol for PDCD2L antibody 68249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols