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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDCD2L Monoclonal antibody, PBS Only

PDCD2L Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G12C4

Cat no : 68249-1-PBS

Synonyms

PDCD2L, programmed cell death 2 like

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDCD2L Monoclonal antibody (68249-1-Ig)

    WB analysis using 68249-1-Ig (same clone as 68249-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68249-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDCD2L Monoclonal antibody (68249-1-Ig)

    WB analysis using 68249-1-Ig (same clone as 68249-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68249-1-Ig (PDCD2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68249-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PDCD2L Monoclonal antibody (68249-1-Ig)

    IF Staining of HEK-293 using 68249-1-Ig (same clone as 68249-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PDCD2L antibody (68249-1-Ig, Clone: 2G12C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68249-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68249-1-PBS targets PDCD2L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDCD2L fusion protein Ag30807 相同性解析による交差性が予測される生物種
Full Name programmed cell death 2-like
Calculated molecular weight 358 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC006146
Gene symbol PDCD2L
Gene ID (NCBI) 84306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PDCD2L was aberrantly expressed in multiple types of human cancers, and associated with clinical stage and molecular subtype and PDCD2L could be served as a biomarker of CRC (PMID: 35216602).