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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK293 cells using PDCD4 Monoclonal antibody (66100-1-Ig)

    WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

    WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PDCD4 Monoclonal antibody (66100-1-Ig)

    WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PDCD4 Monoclonal antibody (66100-1-Ig)

    WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PDCD4 Monoclonal antibody (66100-1-Ig)

    WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PDCD4 Monoclonal antibody (66100-1-Ig)

    WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PDCD4 Monoclonal antibody (66100-1-Ig)

    WB analysis of Jurkat using 66100-1-Ig (same clone as 66100-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDCD4 Monoclonal antibody (66100-1-Ig)

    IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PDCD4 Monoclonal antibody (66100-1-Ig)

    IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PDCD4 Monoclonal antibody, PBS Only

PDCD4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66100-1-PBS
Clone No.1F1A11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66100-1-PBS targets PDCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDCD4 fusion protein Ag19239 相同性解析による交差性が予測される生物種
Full Name programmed cell death 4 (neoplastic transformation inhibitor)
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC026104
Gene Symbol PDCD4
Gene ID (NCBI) 27250
RRIDAB_2881499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53EL6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.