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PDCD4 Monoclonal antibody

PDCD4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F1A11

Cat no : 66100-1-Ig

Synonyms

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a


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Tested Applications

Positive WB detected inHeLa cells, HEK293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66100-1-Ig targets PDCD4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDCD4 fusion protein Ag19239 相同性解析による交差性が予測される生物種
Full Name programmed cell death 4 (neoplastic transformation inhibitor)
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC026104
Gene symbol PDCD4
Gene ID (NCBI) 27250
RRIDAB_2881499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.

Protocols

Product Specific Protocols
WB protocol for PDCD4 antibody 66100-1-IgDownload protocol
IHC protocol for PDCD4 antibody 66100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.

Authors - Ting Peng
humanWB

Clin Res Hepatol Gastroenterol

Long non-coding RNA LINC00472 suppresses hepatocellular carcinoma cell proliferation, migration and invasion through miR-93-5p/PDCD4 pathway.

Authors - Changyu Chen
humanWB

J Tradit Chin Med

Qilan preparation inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells

Authors - Ding Jiamin