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CoraLite® Plus 488-conjugated PDCD4 Monoclonal antibody

PDCD4 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F1A11

Cat no : CL488-66100

Synonyms

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a


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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66100 targets PDCD4 in WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDCD4 fusion protein Ag19239 相同性解析による交差性が予測される生物種
Full Name programmed cell death 4 (neoplastic transformation inhibitor)
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC026104
Gene symbol PDCD4
Gene ID (NCBI) 27250
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 PDCD4 antibody CL488-66100Download protocol
Standard Protocols
Click here to view our Standard Protocols