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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDE6A Monoclonal antibody

PDE6A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3B4

Cat no : 67832-1-Ig

Synonyms

CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using PDE6A Monoclonal antibody (67832-1-Ig)

    WB analysis of mouse eye using 67832-1-Ig

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse retina tissue using PDE6A Monoclonal antibody (67832-1-Ig)

    WB analysis of mouse retina using 67832-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat eye tissue using PDE6A Monoclonal antibody (67832-1-Ig)

    WB analysis of rat eye using 67832-1-Ig

    rat eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue, mouse retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67832-1-Ig targets PDE6A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDE6A fusion protein Ag16741 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 6A, cGMP-specific, rod, alpha
Calculated molecular weight 860 aa, 100 kDa
Observed molecular weight100 kDa
GenBank accession numberBC035909
Gene symbol PDE6A
Gene ID (NCBI) 5145
RRIDAB_2918594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.

Protocols

Product Specific Protocols
WB protocol for PDE6A antibody 67832-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols