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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using PDE6A Monoclonal antibody (67832-1-Ig)

    WB analysis of mouse eye using 67832-1-Ig (same clone as 67832-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67832-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse retina tissue using PDE6A Monoclonal antibody (67832-1-Ig)

    WB analysis of mouse retina using 67832-1-Ig (same clone as 67832-1-PBS)

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67832-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat eye tissue using PDE6A Monoclonal antibody (67832-1-Ig)

    WB analysis of rat eye using 67832-1-Ig (same clone as 67832-1-PBS)

    rat eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67832-1-PBS in a different storage buffer formulation.

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PDE6A Monoclonal antibody, PBS Only

PDE6A Monoclonal Antibody for WB, ELISA
Cat No. 67832-1-PBS
Clone No.1B3B4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67832-1-PBS targets PDE6A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDE6A fusion protein Ag16741 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 6A, cGMP-specific, rod, alpha
Calculated molecular weight 860 aa, 100 kDa
Observed molecular weight100 kDa
GenBank accession numberBC035909
Gene Symbol PDE6A
Gene ID (NCBI) 5145
RRIDAB_2918594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16499
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.