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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01256-4

    Sandwich ELISA standard curve of MP01256-4

    Sandwich ELISA standard curve of MP01256-4, Human PDGFR alpha/CD140a Recombinant Matched Antibody Pair - PBS only. 84383-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00003. 98141-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01256-4

    Sample test of MP01256-4

    The mean PDGFR alpha/CD140a concentration was determined to be 2.6 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load and 7.0 ng/mL in human placenta cell extract based on a 1.7 mg/mL extract load.

  • Sample test of MP01256-4

    Sample test of MP01256-4

    Serum of six individual healthy human donors was measured. The PDGFR alpha/CD140a concentration of detected samples was determined to be 2,557.7 pg/mL with a range of 1,472.3-4,545.6 pg/mL.

  • Flow cytometry (FC) experiment of MG-63 cells using Anti-Human PDGFR alpha/CD140a Rabbit Recombinant A (98141-1-RR)

    FC experiment of MG-63 using 98141-1-RR (same clone as 98141-1-PBS)

    1x10^6 MG-63 cells (left) or A-172 cells (right) were surface stained with 0.25 ug Anti-Human PDGFR alpha/CD140a Rabbit Recombinant Antibody (98141-1-RR, Clone: 241658H4) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98141-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01256-1

    Cytometric bead array standard curve of MP01256-1

    Cytometric bead array standard curve of MP01256-1, PDGFR alpha/CD140a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84383-5-PBS. Detection antibody: 98141-1-PBS. Standard: RP00003. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 98141-1-RR

    Affinity and Kinetic Characterization of 98141-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98141-1-RR against Human PDGFR alpha/CD140a were performed. The affinity constant is 2.26 nM.

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PDGFR alpha/CD140a Recombinant antibody, PBS Only (Detector)

PDGFR alpha/CD140a Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98141-1-PBS
Clone No.241658H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98141-1-PBS targets PDGFR alpha/CD140a as part of a matched antibody pair:

MP01256-1: 84383-5-PBS capture and 98141-1-PBS detection (validated in Cytometric bead array)

MP01256-4: 84383-7-PBS capture and 98141-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name platelet-derived growth factor receptor, alpha polypeptide
Calculated molecular weight 123 kDa
GenBank accession numberBC015186
Gene Symbol PDGFR alpha
Gene ID (NCBI) 5156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16234
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.