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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PDHB Polyclonal antibody

PDHB Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14744

Synonyms

PHE1B, PDHE1-B, PDHE1B, EC:1.2.4.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PDHB Polyclonal anti (CL488-14744)

    IF Staining of HeLa using CL488-14744

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PDHB antibody (CL488-14744) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PDHB Polyclonal anti (CL488-14744)

    FC experiment of HeLa using CL488-14744

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PDHB Polyclonal antibody (CL488-14744)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14744 targets PDHB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDHB fusion protein Ag6442 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase (lipoamide) beta
Calculated molecular weight 39 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000439
Gene symbol PDHB
Gene ID (NCBI) 5162
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDHB(Pyruvate dehydrogenase E1 component subunit beta, mitochondrial) is also named as PHE1B. The enzyme, which is found in mitochondria, is one of the component enzymes of the pyruvate dehydrogenase multienzyme complex (PDH). It catalyzes the first reaction of an oxidative decarboxylation sequence converting pyruvate to acetyl-CoA and CO(2)(PMID:3422424). Defects in PDHB are the cause of pyruvate dehydrogenase E1-beta deficiency (PDHBD)(PMID:15138885).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PDHB antibody CL488-14744Download protocol
FC protocol for CL Plus 488 PDHB antibody CL488-14744Download protocol
Standard Protocols
Click here to view our Standard Protocols