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Species Reactivity

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  • Featured Product
  • KD/KO Validated

PDI Polyclonal antibody

PDI Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 11245-1-AP

Synonyms

P4HB, GIT, ERBA2L, EC:5.3.4.1, DSI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDI Polyclonal antibody (11245-1-AP)

    WB analysis using 11245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11245-1-AP (PDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PDI Polyclonal antibody (11245-1-AP)

    WB analysis of HEK-293 using 11245-1-AP

    WB result of PDI antibody (11245-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PDI Polyclonal antibody (11245-1-AP)

    IP experiment of HepG2 using 11245-1-AP

    IP result of anti-PDI (IP:11245-1-AP, 4ug; Detection:11245-1-AP 1:2000) with HepG2 cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PDI Polyclonal antibody (11245-1-AP)

    IHC staining of human prostate cancer using 11245-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using PDI Polyclonal antibody (11245-1-AP)

    IHC staining of human colon using 11245-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDI Polyclonal antibody (11245-1-AP)

    IF Staining of HepG2 using 11245-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDI antibody (11245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

  • Flow cytometry (FC) experiment of HeLa cells using PDI Polyclonal antibody (11245-1-AP)

    FC experiment of HeLa using 11245-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PDI (11245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, HepG2 cells, mouse heart tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11245-1-AP targets PDI in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, pig, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDI fusion protein Ag1747 相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014504
Gene symbol PDI
Gene ID (NCBI) 5034
RRIDAB_2298937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Protocols

Product Specific Protocols
WB protocol for PDI antibody 11245-1-APDownload protocol
IHC protocol for PDI antibody 11245-1-APDownload protocol
IF protocol for PDI antibody 11245-1-APDownload protocol
IP protocol for PDI antibody 11245-1-APDownload protocol
FC protocol for PDI antibody 11245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
View Article
human,mouseIHC

Nature

Heart repair by reprogramming non-myocytes with cardiac transcription factors.

Authors - Song Kunhua K
View Article
humanIF

J Thromb Haemost

VWF-Gly2752Ser, a novel non-cysteine substitution variant in the CK domain, exhibits severe secretory impairment by hampering C-terminal dimer formation.

Authors - Shuichi Okamoto
View Article
IF

Mol Cell

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

Authors - Linyu Sun
View Article
humanIHC, WB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
View Article
mouseIF

Sci Adv

Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry.

Authors - Katsutoshi Mizuno
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MSCs
PDI Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:11245-1-AP)
FH

Anastasia (Verified Customer) (08-07-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Zebrafish embryos
PDI Antibody Western Blot validation (1:500 dilution) in Zebrafish embryos (Cat no:11245-1-AP)