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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated PDI Polyclonal antibody

PDI Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-11245

Synonyms

P4HB, GIT, ERBA2L, EC:5.3.4.1, DSI

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated PDI Polyclonal antib (CL647-11245)

    IF Staining of HepG2 using CL647-11245

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 PDI antibody (CL647-11245) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated PDI Polyclonal antib (CL647-11245)

    FC experiment of HeLa using CL647-11245

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human PDI (CL647-11245) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-11245 targets PDI in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDI fusion protein Ag1747 相同性解析による交差性が予測される生物種
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014504
Gene symbol PDI
Gene ID (NCBI) 5034
RRIDAB_2920216
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 PDI antibody CL647-11245Download protocol
FC protocol for CL Plus 647 PDI antibody CL647-11245Download protocol
Standard Protocols
Click here to view our Standard Protocols