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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERp57/ERp60 Monoclonal antibody

ERp57/ERp60 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G4G7

Cat no : 66423-1-Ig

Synonyms

ERp57, PDIA3, ER60, EC:5.3.4.1, Disulfide isomerase ER-60

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    WB analysis using 66423-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:9700 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    WB analysis of HeLa using 66423-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    WB analysis of HEK-293 using 66423-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    WB analysis of Jurkat using 66423-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    WB analysis of RAW 264.7 using 66423-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    IHC staining of human lung cancer using 66423-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    IHC staining of human lung cancer using 66423-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    IHC staining of human liver cancer using 66423-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    IHC staining of human liver cancer using 66423-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66423-1-Ig (ERp57/ERp60 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    IF Staining of HepG2 using 66423-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ERp57/ERp60 Monoclonal antibody (66423-1-Ig)

    IF Staining of HEK-293 using 66423-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ERp57/ERp60 antibody (66423-1-Ig, Clone: 3G4G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, LNCaP cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66423-1-Ig targets ERp57/ERp60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERp57/ERp60 fusion protein Ag8741 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 3
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57 kDa, 60 kDa
GenBank accession numberBC014433
Gene symbol PDIA3
Gene ID (NCBI) 2923
RRIDAB_2881795
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protocols

Product Specific Protocols
WB protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
IHC protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
IF protocol for ERp57/ERp60 antibody 66423-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols