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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ERp57/ERp60 Monoclonal antibody

ERp57/ERp60 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G4G7

Cat no : CL594-66423

Synonyms

58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CoraLite®594-conjugated ERp57/ERp60 Monoclonal ant (CL594-66423)

    IF Staining of U2OS using CL594-66423

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®594 ERp57/ERp60 antibody (CL594-66423, Clone: 3G4G7 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66423 targets ERp57/ERp60 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERp57/ERp60 fusion protein Ag8741 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 3
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57 kDa, 60 kDa
GenBank accession numberBC014433
Gene symbol PDIA3
Gene ID (NCBI) 2923
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.

Protocols

Product Specific Protocols
IF protocol for CL594 ERp57/ERp60 antibody CL594-66423Download protocol
Standard Protocols
Click here to view our Standard Protocols