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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated PDIA6 Monoclonal antibody (CL594-66669)

    IF Staining of HepG2 using CL594-66669

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66669 (PDIA6 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated PDIA6 Monoclonal antibody (CL594-66669)

    FC experiment of HepG2 using CL594-66669

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PDIA6 (CL594-66669, Clone:1G2D4) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated PDIA6 Monoclonal antibody

PDIA6 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66669
Clone No.1G2D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

Protein disulfide isomerase P5, Protein disulfide isomerase A6, P5, ERp5, ER protein 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66669 targets PDIA6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDIA6 fusion protein Ag27608 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 6
Calculated molecular weight 48 kDa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC001312
Gene Symbol PDIA6
Gene ID (NCBI) 10130
RRIDAB_2883612
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15084
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.

Protocols

Product Specific Protocols
IF protocol for CL594 PDIA6 antibody CL594-66669Download protocol
Standard Protocols
Click here to view our Standard Protocols