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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDLIM5 Monoclonal antibody (67651-1-Ig)

    WB analysis using 67651-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67651-1-Ig (PDLIM5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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PDLIM5 Monoclonal antibody

PDLIM5 Monoclonal Antibody for WB, ELISA
Cat No. 67651-1-Ig
Clone No.1H9C9

Host / Isotype

Mouse / IgM

Reactivity

Human

Applications

WB, ELISA

Synonyms

ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman heart tissue, human platelets

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67651-1-Ig targets PDLIM5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen PDLIM5 fusion protein Ag25059 相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 5
Calculated molecular weight 64 kDa
Observed molecular weight 63-69 kDa
GenBank accession numberBC008741
Gene Symbol PDLIM5
Gene ID (NCBI) 10611
RRIDAB_2882850
Conjugate Unconjugated
Form Liquid
Purification MethodEuglobulin precipitation
UNIPROT IDQ96HC4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression.

Protocols

Product Specific Protocols
WB protocol for PDLIM5 antibody 67651-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols