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CD31 抗体 146 文献

Rabbit Polyclonal| カタログ番号: 11265-1-AP

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, ELISA
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Images See all

CD31 Antibody WB human peripheral blood platelets 11265-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD31 Antibody IHC human hepatocirrhosis tissue 11265-1-AP

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens).

CD31 Antibody IHC human hepatocirrhosis tissue 11265-1-AP

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens).

CD31 Antibody IP Jurkat cells 11265-1-AP

IP Result of anti-PECAM1,CD31 (IP:11265-1-AP, 5ug; Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug.

CD31 Antibody IF human tonsillitis tissue 11265-1-AP

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 11265-1-AP (CD31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CD31 Antibody FC HUVEC cells 11265-1-AP

1X10^6 HUVEC cells were stained with 0.2ug CD31 antibody (11265-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

種特異性:
human, monkey
WB ポジティブ:
human peripheral blood platelets, HUVEC cells, Jurkat cells, THP-1 cells
IP ポジティブ:
Jurkat cells
IHC ポジティブ:
human hepatocirrhosis tissue, human tonsillitis tissue, human liver cancer tissue, human gliomas tissue, human renal cell carcinoma tissue, human kidney tissue
IF ポジティブ:
human tonsillitis tissue
FC ポジティブ:
HUVEC cells
推奨希釈倍率:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:800-1:4000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
CD31 fusion protein Ag1787
正式名:
platelet/endothelial cell adhesion molecule
計算分子量:
83 kDa
検出分子量:
120-130 kDa
GenBank accession number:
BC022512
Gene ID (NCBI):
5175
遺伝子シンボル:
PECAM1
別名:
CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

背景説明

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).


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