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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated CD31 Polyclonal antibody

CD31 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11265

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

Validation Data Gallery

Applications:
  • IF Staining of human breast cancer using CL594-11265

    IF Staining of human breast cancer using CL594-11265

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200, CoraLite®488 Cytokeratin 7 antibody (CL488-15539, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta).

  • IF Staining of human breast cancer using CL594-11265

    IF Staining of human breast cancer using CL594-11265

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200, CoraLite®488 Cytokeratin 7 antibody (CL488-15539, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta).

  • IF Staining of HUVEC using CL594-11265

    IF Staining of HUVEC using CL594-11265

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-11265

    IF Staining of human tonsillitis using CL594-11265

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-11265

    IF Staining of human tonsillitis using CL594-11265

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-11265) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman breast cancer tissue, human tonsillitis tissue, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11265-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

CL594-11265 targets CD31 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787 相同性解析による交差性が予測される生物種
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
GenBank accession numberBC022512
Gene symbol PECAM1
Gene ID (NCBI) 5175
RRIDAB_2919762
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

Product Specific Protocols
IF protocol for CL594 CD31 antibody CL594-11265Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Stem Cell Res Ther

Cell heterogeneity, rather than the cell storage solution, affects the behavior of mesenchymal stem cells in vitro and in vivo.

Authors - Yong-Hong Wang
View Article