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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Serpin F1/PEDF Monoclonal antibody

Serpin F1/PEDF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C5C8

Cat no : 66554-1-Ig

Synonyms

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Serpin F1/PEDF Monoclonal antibody (66554-1-Ig)

    WB analysis of human plasma using 66554-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig plasma using Serpin F1/PEDF Monoclonal antibody (66554-1-Ig)

    WB analysis of pig plasma using 66554-1-Ig

    pig plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human blood using Serpin F1/PEDF Monoclonal antibody (66554-1-Ig)

    WB analysis of human blood using 66554-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman plasma tissue, pig plasma tissue, human blood tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66554-1-Ig targets Serpin F1/PEDF in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Serpin F1/PEDF fusion protein Ag26660 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC013984
Gene symbol PEDF
Gene ID (NCBI) 5176
RRIDAB_2881916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

Protocols

Product Specific Protocols
WB protocol for Serpin F1/PEDF antibody 66554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols