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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Serpin F1/PEDF Monoclonal antibody (66554-1-Ig)

    WB analysis of human plasma using 66554-1-Ig (same clone as 66554-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig plasma using Serpin F1/PEDF Monoclonal antibody (66554-1-Ig)

    WB analysis of pig plasma using 66554-1-Ig (same clone as 66554-1-PBS)

    pig plasma were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human blood using Serpin F1/PEDF Monoclonal antibody (66554-1-Ig)

    WB analysis of human blood using 66554-1-Ig (same clone as 66554-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66554-1-Ig (PEDF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66554-1-PBS in a different storage buffer formulation.

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Serpin F1/PEDF Monoclonal antibody, PBS Only

Serpin F1/PEDF Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66554-1-PBS
Clone No.2C5C8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, Indirect ELISA

Synonyms

PIG35, PEDF, EPC-1, EPC 1, Cell proliferation-inducing gene 35 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66554-1-PBS targets Serpin F1/PEDF in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Serpin F1/PEDF fusion protein Ag26660 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46-50 kDa
GenBank accession numberBC013984
Gene Symbol PEDF
Gene ID (NCBI) 5176
RRIDAB_2881916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36955
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.