Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse liver tissue, human liver cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 21 publications below |
| IHC | See 5 publications below |
| IF | See 44 publications below |
Product Information
10594-1-AP targets PEX14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, rat, monkey, chicken, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PEX14 fusion protein Ag0932 相同性解析による交差性が予測される生物種 |
| Full Name | peroxisomal biogenesis factor 14 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC006327 |
| Gene Symbol | PEX14 |
| Gene ID (NCBI) | 5195 |
| RRID | AB_2252194 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75381 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX14 antibody 10594-1-AP | Download protocol |
| IHC protocol for PEX14 antibody 10594-1-AP | Download protocol |
| IF protocol for PEX14 antibody 10594-1-AP | Download protocol |
| IP protocol for PEX14 antibody 10594-1-AP | Download protocol |
| FC protocol for PEX14 antibody 10594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. | ||
Nat Cell Biol Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties | ||