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  • Featured Product
  • KD/KO Validated

PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10594-1-AP

Synonyms

PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PEX14 Polyclonal antibody (10594-1-AP)

    WB analysis of HEK-293 using 10594-1-AP

    WB result of PEX14 antibody (10594-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX14 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PEX14 Polyclonal antibody (10594-1-AP)

    WB analysis using 10594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IP experiment of mouse liver using 10594-1-AP

    IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IHC staining of mouse liver using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IHC staining of mouse liver using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IHC staining of human liver cancer using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IHC staining of human liver cancer using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IHC staining of human cervical cancer using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PEX14 Polyclonal antibody (10594-1-AP)

    IHC staining of human cervical cancer using 10594-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of HeLa using 10594-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of HepG2 using 10594-1-AP

    Immunofluorescent analysis of (-20°C Ethanol ) fixed HepG2 cells using 10594-1-AP (PEX14 Antibody, Green) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L). 66009-1-Ig (Beta actin antibody, Red) and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L). DAPI, for Blue..

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of HepG2 using 10594-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10594-1-AP (PEX14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of NIH/3T3 using 10594-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PEX14 antibody (10594-1-AP) at dilution of 1:150 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of C2C12 using 10594-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using PEX14 antibody (10594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of HeLa using 10594-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of COS7 cells using PEX14 Polyclonal antibody (10594-1-AP)

    IF Staining of COS7 cells using 10594-1-AP

    The antibody 10594-1-AP stain the Peroxisomes of the COS7 cell transfected with YFP-Lpx1. IF result in Paper with PMID:21741480, Dr. Sven Thoms.

  • Flow cytometry (FC) experiment of HeLa cells using PEX14 Polyclonal antibody (10594-1-AP)

    FC experiment of HeLa using 10594-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (10594-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, SKOV-3 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10594-1-AP targets PEX14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, monkey, chicken, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX14 fusion protein Ag0932 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC006327
Gene symbol PEX14
Gene ID (NCBI) 5195
RRIDAB_2252194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Protocols

Product Specific Protocols
WB protocol for PEX14 antibody 10594-1-APDownload protocol
IHC protocol for PEX14 antibody 10594-1-APDownload protocol
IF protocol for PEX14 antibody 10594-1-APDownload protocol
IP protocol for PEX14 antibody 10594-1-APDownload protocol
FC protocol for PEX14 antibody 10594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
View Article
mouseIF

Cell

A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart.

Authors - José A Nicolás-Ávila
View Article
humanIF

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
View Article
mouseIF

Mol Cell

Drp1 Tubulates the ER in a GTPase-Independent Manner.

Authors - Yoshihiro Adachi
View Article
mouseWB, IHC

Cell Metab

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Authors - Katharina M Walter
View Article
humanWB

Nat Chem Biol

Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.

Authors - Christopher F Bennett
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

PAULA (Verified Customer) (07-12-2023)

I tested this antibody on THP1 cells (20ug protein extract) at a 1:1000 dilution for WB and although I saw a band around the molecular weight of the protein, different bands were also detected suggesting some non-specific binding.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: THP1 cell line
PEX14 Antibody Western Blot validation (1:1000 dilution) in THP1 cell line (Cat no:10594-1-AP)
FH

Tim (Verified Customer) (04-09-2021)

20ug cell lysate loaded and separated by SDS-PAGE prior to transfer. Antibody incubation overnight in cold room.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HEK293
PEX14 Antibody Western Blot validation (1/2000 dilution) in HEK293 (Cat no:10594-1-AP)
FH

Tom (Verified Customer) (03-30-2021)

HEK293 cells fixed in methanol at -20 degrees. PEX14 antibody visualized using anti-rabbit 594 (red) secondary antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HEK293
PEX14 Antibody Immunofluorescence validation (1:100 dilution) in HEK293 (Cat no:10594-1-AP)
FH

Chun (Verified Customer) (12-05-2019)

A good antibody

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa cells