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Biotin-conjugated PEX14 Polyclonal antibody
PEX14 Polyclonal Antibody for IHC, IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, monkey
Applications
IHC, IF/ICC
Conjugate
Biotin
Cat no : Biotin-10594
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-10594 targets PEX14 in IHC, IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PEX14 fusion protein Ag0932 相同性解析による交差性が予測される生物種 |
Full Name | peroxisomal biogenesis factor 14 |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 57 kDa |
GenBank accession number | BC006327 |
Gene symbol | PEX14 |
Gene ID (NCBI) | 5195 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin PEX14 antibody Biotin-10594 | Download protocol |
IF protocol for Biotin PEX14 antibody Biotin-10594 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |