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  • Featured Product
  • KD/KO Validated

Biotin-conjugated PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for IHC, IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IHC, IF/ICC

Conjugate

Biotin

Cat no : Biotin-10594

Synonyms

PTS1 receptor docking protein, Pex14p, PEX, Peroxin-14, Peroxin 14

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using Biotin-conjugated PEX14 Polyclonal antibody (Biotin-10594)

    IHC staining of mouse liver using Biotin-10594

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using Biotin-10594 (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Biotin-conjugated PEX14 Polyclonal antibody (Biotin-10594)

    IHC staining of mouse liver using Biotin-10594

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using Biotin-10594 (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Biotin-conjugated PEX14 Polyclonal antibody (Biotin-10594)

    IF Staining of HepG2 using Biotin-10594

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Biotin PEX14 antibody (Biotin-10594) at dilution of 1:200 and Alexa Fluor 594 Streptavidin.

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Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-10594 targets PEX14 in IHC, IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX14 fusion protein Ag0932 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC006327
Gene symbol PEX14
Gene ID (NCBI) 5195
Conjugate Biotin
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Protocols

Product Specific Protocols
IHC protocol for Biotin PEX14 antibody Biotin-10594Download protocol
IF protocol for Biotin PEX14 antibody Biotin-10594Download protocol
Standard Protocols
Click here to view our Standard Protocols