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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEX14 Recombinant antibody

PEX14 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

7E6

Cat no : 80234-1-RR

Synonyms

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEX14 Recombinant antibody (80234-1-RR)

    WB analysis using 80234-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80234-1-RR (PEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using PEX14 Recombinant antibody (80234-1-RR)

    WB analysis of LNCaP using 80234-1-RR

    LNCaP cells were subjected to SDS PAGE followed by western blot with 80234-1-RR (PEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX14 Recombinant antibody (80234-1-RR)

    IHC staining of human liver cancer using 80234-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX14 Recombinant antibody (80234-1-RR)

    IHC staining of human liver cancer using 80234-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PEX14 Recombinant antibody (80234-1-RR)

    IHC staining of human cervical cancer using 80234-1-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PEX14 Recombinant antibody (80234-1-RR)

    IHC staining of human cervical cancer using 80234-1-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX14 Recombinant antibody (80234-1-RR)

    IF Staining of HeLa using 80234-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (80234-1-RR, Clone: 7E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80234-1-RR

    Affinity and Kinetic Characterization of 80234-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80234-1-RR against Human PEX14 were performed. The affinity constant is 1.96 nM.

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Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80234-1-RR targets PEX14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEX14 fusion protein Ag0932 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight57 kDa
GenBank accession numberBC006327
Gene symbol PEX14
Gene ID (NCBI) 5195
RRIDAB_2918880
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Protocols

Product Specific Protocols
WB protocol for PEX14 antibody 80234-1-RRDownload protocol
IHC protocol for PEX14 antibody 80234-1-RRDownload protocol
IF protocol for PEX14 antibody 80234-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols