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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PEX14 Recombinant antibody

PEX14 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

7E6

Cat no : CL594-80234

Synonyms

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated PEX14 Recombinant antibody (CL594-80234)

    IF Staining of HeLa using CL594-80234

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-80234, Clone: 7E6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated PEX14 Recombinant antibody (CL594-80234)

    FC experiment of HeLa using CL594-80234

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PEX14 (CL594-80234, Clone:7E6) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80234 targets PEX14 in IF/ICC, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEX14 fusion protein Ag0932 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight57 kDa
GenBank accession numberBC006327
Gene symbol PEX14
Gene ID (NCBI) 5195
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Protocols

Product Specific Protocols
IF protocol for CL594 PEX14 antibody CL594-80234Download protocol
FC protocol for CL594 PEX14 antibody CL594-80234Download protocol
Standard Protocols
Click here to view our Standard Protocols