Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, HEK-293 cells, fetal human brain tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66309-1-Ig targets PEX5 in WB, IHC, ELISA applications and shows reactivity with human, pig, rat samples.
| Tested Reactivity | human, pig, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PEX5 fusion protein Ag3268 相同性解析による交差性が予測される生物種 |
| Full Name | peroxisomal biogenesis factor 5 |
| Calculated molecular weight | 631 aa, 70 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC010621 |
| Gene Symbol | PEX5 |
| Gene ID (NCBI) | 5830 |
| RRID | AB_2881691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P50542 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX5 antibody 66309-1-Ig | Download protocol |
| IHC protocol for PEX5 antibody 66309-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|