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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of HEK-293 using 66309-1-Ig (same clone as 66309-1-PBS)

    WB result of PEX5 antibody (66309-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells. This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of pig brain using 66309-1-Ig (same clone as 66309-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    WB analysis of fetal human brain using 66309-1-Ig (same clone as 66309-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66309-1-Ig (PEX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig (same clone as 66309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human liver using 66309-1-Ig (same clone as 66309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig (same clone as 66309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Monoclonal antibody (66309-1-Ig)

    IHC staining of human kidney using 66309-1-Ig (same clone as 66309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66309-1-Ig (PEX5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66309-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PEX5 Monoclonal antibody, PBS Only

PEX5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66309-1-PBS
Clone No.1C2C3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Cell and organelle markers, Peroxin 5, Peroxisome Marker, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66309-1-PBS targets PEX5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEX5 fusion protein Ag3268 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 5
Calculated molecular weight 631 aa, 70 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC010621
Gene Symbol PEX5
Gene ID (NCBI) 5830
RRIDAB_2881691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP50542
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder.