Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13763-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13763-1-AP
WB result of PFKFB3-Specific antibody (13763-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKFB3-Specific transfected HEK-293 cells.
WB analysis of mouse heart using 13763-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 13763-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13763-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 13763-1-AP
IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:500) with mouse spleen tissue lysate 2440 ug.
IP experiment of mouse spleen using 13763-1-AP
IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.
IP experiment of mouse spleen using 13763-1-AP
IP result of anti-PFKFB3 (IP:13763-1-AP, 4ug; Detection:13763-1-AP 1:300) with mouse spleen tissue lysate 3920 ug.
IHC staining of human liver cancer using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 13763-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PFKFB3-Specific antibody (13763-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PFKFB3 Polyclonal antibody

PFKFB3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13763-1-AP

Publications(127)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

IPFK2, Fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, 6-phosphofructo-2-kinase, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (127)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse thymus tissue, mouse heart tissue, A431 cells, HeLa cells, Jurkat cells, mouse spleen tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human liver cancer tissue, human kidney tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 19 publications below
WB	See 115 publications below
IHC	See 27 publications below
IF	See 17 publications below
IP	See 4 publications below
FC	See 1 publications below
CoIP	See 2 publications below

Product Information

13763-1-AP targets PFKFB3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PFKFB3 fusion protein Ag4744 相同性解析による交差性が予測される生物種
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Calculated molecular weight	520 aa, 60 kDa
Observed molecular weight	58 kDa
GenBank accession number	BC040482
Gene Symbol	PFKFB3
Gene ID (NCBI)	5209
RRID	AB_2162854
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16875
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. Its synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5.

Protocols

Product Specific Protocols
WB protocol for PFKFB3 antibody 13763-1-AP	Download protocol
IHC protocol for PFKFB3 antibody 13763-1-AP	Download protocol
IF protocol for PFKFB3 antibody 13763-1-AP	Download protocol
IP protocol for PFKFB3 antibody 13763-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature FGF-dependent metabolic control of vascular development. Authors - Pengchun Yu View Article
human	WB	Cell Role of PFKFB3-Driven Glycolysis in Vessel Sprouting. Authors - De Bock Katrien K View Article
mouse	WB	Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption Authors - Qinqin He View Article
human	WB	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li View Article
human	WB,IP	Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Authors - Mi Kyung Park View Article
mouse	WB	Circulation Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth. Authors - Andrew A Gibb View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13763-1-AP

WB analysis of HEK-293 using 13763-1-AP

WB analysis of mouse heart using 13763-1-AP

WB analysis of A431 using 13763-1-AP

WB analysis of mouse thymus using 13763-1-AP

IP experiment of mouse spleen using 13763-1-AP

IP experiment of mouse spleen using 13763-1-AP

IP experiment of mouse spleen using 13763-1-AP

IHC staining of human liver cancer using 13763-1-AP

IHC staining of human liver cancer using 13763-1-AP

IHC staining of human liver using 13763-1-AP

IHC staining of human liver using 13763-1-AP

IHC staining of human kidney using 13763-1-AP

IHC staining of human kidney using 13763-1-AP

IF Staining of HepG2 using 13763-1-AP

PFKFB3 Polyclonal antibody

PFKFB3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FGF-dependent metabolic control of vascular development.

Role of PFKFB3-Driven Glycolysis in Vessel Sprouting.

Acetate enables metabolic fitness and cognitive performance during sleep disruption

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth.