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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PFKFB3-Specific Polyclonal antibody

PFKFB3-Specific Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13763

Synonyms

6PF 2 K/Fru 2,6 P2ase 3, FLJ37326, IPFK2, PFK/FBPase 3, PFK2, PFKFB3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated PFKFB3-Specific Poly (CL488-13763)

    IF Staining of HepG2 using CL488-13763

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PFKFB3-Specific antibody (CL488-13763) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13763 targets PFKFB3-Specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB3-Specific fusion protein Ag4744 相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC040482
Gene symbol PFKFB3
Gene ID (NCBI) 5209
RRIDAB_2919078
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PFKFB3-Specific antibody CL488-13763Download protocol
Standard Protocols
Click here to view our Standard Protocols