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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PFKP Monoclonal antibody

PFKP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H4D4

Cat no : CL488-68129

Synonyms

6 phosphofructokinase type C, FLJ40226, PFK C, PFKF, PFKP, Phosphofructokinase 1, phosphofructokinase, platelet, Phosphohexokinase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PFKP Monoclonal anti (CL488-68129)

    IF Staining of HeLa using CL488-68129

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PFKP antibody (CL488-68129, Clone: 1H4D4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68129 targets PFKP in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PFKP fusion protein Ag25206 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, platelet
Calculated molecular weight 784 aa, 86 kDa
Observed molecular weight80-86 kDa
GenBank accession numberBC029138
Gene symbol PFKP
Gene ID (NCBI) 5214
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PFKP antibody CL488-68129Download protocol
Standard Protocols
Click here to view our Standard Protocols