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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PGAM5 Polyclonal antibody

PGAM5 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28445

Synonyms

Serine/threonine-protein phosphatase PGAM5, mitochondrial, EC:3.1.3.16, BXLBv68, Bcl-XL-binding protein v68

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated PGAM5 Polyclonal ant (CL488-28445)

    IF Staining of MCF-7 using CL488-28445

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PGAM5 antibody (CL488-28445) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated PGAM5 Polyclonal ant (CL488-28445)

    FC experiment of MCF-7 using CL488-28445

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pgam5 Polyclonal Antibody (CL488-28445)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28445 targets PGAM5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGAM5 fusion protein Ag28195 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate mutase family member 5
Calculated molecular weight 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC008196
Gene symbol PGAM5
Gene ID (NCBI) 192111
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PGAM5 antibody CL488-28445Download protocol
FC protocol for CL Plus 488 PGAM5 antibody CL488-28445Download protocol
Standard Protocols
Click here to view our Standard Protocols