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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGD Monoclonal antibody

PGD Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10G4

Cat no : 67916-1-Ig

Synonyms

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGD Monoclonal antibody (67916-1-Ig)

    WB analysis using 67916-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using PGD Monoclonal antibody (67916-1-Ig)

    WB analysis using 67916-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PGD Monoclonal antibody (67916-1-Ig)

    IF Staining of HepG2 using 67916-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67916-1-Ig targets PGD in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGD fusion protein Ag6871 相同性解析による交差性が予測される生物種
Full Name phosphogluconate dehydrogenase
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa, 45 kDa
GenBank accession numberBC000368
Gene symbol PGD
Gene ID (NCBI) 5226
RRIDAB_2918670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)

Protocols

Product Specific Protocols
WB protocol for PGD antibody 67916-1-IgDownload protocol
IF protocol for PGD antibody 67916-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols