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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGD Monoclonal antibody (67916-1-Ig)

    WB analysis using 67916-1-Ig (same clone as 67916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PGD Monoclonal antibody (67916-1-Ig)

    WB analysis using 67916-1-Ig (same clone as 67916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67916-1-Ig (PGD antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PGD Monoclonal antibody (67916-1-Ig)

    IF Staining of HepG2 using 67916-1-Ig (same clone as 67916-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PGD antibody (67916-1-Ig, Clone: 2A10G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67916-1-PBS in a different storage buffer formulation.

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PGD Monoclonal antibody, PBS Only

PGD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67916-1-PBS
Clone No.2A10G4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

6PGD, PGD, PGDH, phosphogluconate dehydrogenase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67916-1-PBS targets PGD in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGD fusion protein Ag6871 相同性解析による交差性が予測される生物種
Full Name phosphogluconate dehydrogenase
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa, 45 kDa
GenBank accession numberBC000368
Gene Symbol PGD
Gene ID (NCBI) 5226
RRIDAB_2918670
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52209
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PGD, also named as PGDH, belongs to the 6-phosphogluconate dehydrogenase family. PGD catalyses the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate in the context of the oxidative part of the pentose phosphate pathway. PGD is important for the production of NADPH, which is necessary for reductive biosynthesis, such as the formation of lipids and nucleotides, and the activity of enzymes involved in maintaining cell integrity, in combatting oxidative stress and in the first line of immunological defence. (PMID: 35234135)