Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, Jurkat cells, human platelet tissue, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68035-1-Ig targets PGK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PGK1 fusion protein Ag12099 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoglycerate kinase 1 |
| Calculated molecular weight | 417 aa, 45 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC103752 |
| Gene Symbol | PGK1 |
| Gene ID (NCBI) | 5230 |
| RRID | AB_2918777 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| IHC protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| IF protocol for PGK1 antibody 68035-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Oncol Tetraarsenic tetrasulfide triggers ROS-induced apoptosis and ferroptosis in B-cell acute lymphoblastic leukaemia by targeting HK2 |