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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGK1 Monoclonal antibody (68035-1-Ig)

    WB analysis using 68035-1-Ig (same clone as 68035-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68035-1-Ig (PGK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of human liver cancer using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of rat kidney using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of rat kidney using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of rat testis using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of rat testis using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of mouse kidney using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PGK1 Monoclonal antibody (68035-1-Ig)

    IHC staining of mouse kidney using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68035-1-Ig (PGK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PGK1 Monoclonal antibody (68035-1-Ig)

    IF Staining of A431 using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PGK1 Monoclonal antibody (68035-1-Ig)

    IF Staining of HepG2 using 68035-1-Ig (same clone as 68035-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGK1 antibody (68035-1-Ig, Clone: 1B2F3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68035-1-PBS in a different storage buffer formulation.

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PGK1 Monoclonal antibody, PBS Only

PGK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68035-1-PBS
Clone No.1B2F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PRP 2, PGKA, OK/SW-cl.110, EC:2.7.2.3, Cell migration-inducing gene 10 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68035-1-PBS targets PGK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGK1 fusion protein Ag12099 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate kinase 1
Calculated molecular weight 417 aa, 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC103752
Gene Symbol PGK1
Gene ID (NCBI) 5230
RRIDAB_2918777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00558
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3- phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei. PGK1 has 2 isoforms with the molecular mass of 41 and 45 kDa (PMID: 31815035, PMID: 31911580).