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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated PGRMC2 Monoclonal antibody

PGRMC2 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5F10D7

Cat no : CL594-60249

Synonyms

DG6, PGRMC2, PMBP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using CoraLite®594-conjugated PGRMC2 Monoclonal antibody (CL594-60249)

    IF Staining of human cervical cancer using CL594-60249

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 PGRMC2 antibody (CL594-60249, Clone: 5F10D7 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using CoraLite®594-conjugated PGRMC2 Monoclonal antibody (CL594-60249)

    IF Staining of human cervical cancer using CL594-60249

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using CoraLite®594 PGRMC2 antibody (CL594-60249, Clone: 5F10D7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated PGRMC2 Monoclonal antibody (CL594-60249)

    FC experiment of HepG2 using CL594-60249

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PGRMC2 (CL594-60249, Clone:5F10D7) (red), or 0.4 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman cervical cancer tissue
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60249 targets PGRMC2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PGRMC2 fusion protein Ag20077 相同性解析による交差性が予測される生物種
Full Name progesterone receptor membrane component 2
Calculated molecular weight 223 aa, 24 kDa
Observed molecular weight 24+28 kDa
GenBank accession numberBC016692
Gene symbol PGRMC2
Gene ID (NCBI) 10424
RRIDAB_2919905
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).

Protocols

Product Specific Protocols
IF protocol for CL594 PGRMC2 antibody CL594-60249Download protocol
FC protocol for CL594 PGRMC2 antibody CL594-60249Download protocol
Standard Protocols
Click here to view our Standard Protocols