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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PHAX Polyclonal antibody, PBS Only

PHAX Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16481-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PHAX Polyclonal antibody (16481-1-AP)

    WB analysis of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PHAX Polyclonal antibody (16481-1-AP)

    WB analysis of HepG2 using 16481-1-AP (same clone as 16481-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PHAX Polyclonal antibody (16481-1-AP)

    WB analysis of HepG2 using 16481-1-AP (same clone as 16481-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHAX Polyclonal antibody (16481-1-AP)

    IF Staining of HeLa using 16481-1-AP (same clone as 16481-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16481-1-AP (PHAX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16481-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16481-1-PBS targets PHAX in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHAX fusion protein Ag9602 相同性解析による交差性が予測される生物種
Full Name phosphorylated adaptor for RNA export
Calculated molecular weight 394 aa, 44 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC021161
Gene symbol PHAX
Gene ID (NCBI) 51808
RRIDAB_2299663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Small nuclear and small nucleolar RNAs (snRNAs and snoRNAs) are essential components of snRNPs and snoRNPs, and have a critical role in the maturation of, respectively, mRNAs and rRNAs within the nucleus of eukaryotic cells. Complex and specific pathways exist for the assembly of snRNPs and snoRNPs, involving, nucleocytoplasmic transport of snRNAs and intranuclear transport between compartments of snoRNAs. In metazoa, a subset of spliceosomal snRNAs are exported from the nucleus after transcription. This export occurs in a large complex containing a snRNA, the nuclear cap binding complex (CBC), RanGTP, the leucine-rich nuclear export signal receptor CRM1/Xpo1, and the recently identified phosphoprotein PHAX (phosphorylated adaptor for RNA export). PHAX contains a conserved single-stranded nucleic acid binding domain (RNA_GG_bind domain) with no sequence homology with any other known RNA-binding module [PMID:15574332,11333016, 15574332]