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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated PHD2/EGLN1 Monoclonal anti (CL594-66589)

    IF Staining of HEK-293 using CL594-66589

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®®594 PHD2/EGLN1 antibody (CL594-66589, Clone: 1A2F1 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated PHD2/EGLN1 Monoclonal anti (CL594-66589)

    IF Staining of HEK-293 using CL594-66589

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 PHD2/EGLN1 antibody (CL594-66589, Clone: 1A2F1 ) at dilution of 1:200.

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  • KD/KO Validated

CoraLite®594-conjugated PHD2/EGLN1 Monoclonal antibody

PHD2/EGLN1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66589
Clone No.1A2F1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Synonyms

EGLN1, PHD2, EGLN 1, ECYT3, EC:1.14.11.29

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66589 targets PHD2/EGLN1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name egl nine homolog 1 (C. elegans)
Calculated molecular weight 46 kDa
GenBank accession numberNM_022051
Gene Symbol PHD2/EGLN1
Gene ID (NCBI) 54583
RRIDAB_2920011
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZT9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Protocols

Product Specific Protocols
IF protocol for CL594 PHD2/EGLN1 antibody CL594-66589Download protocol
Standard Protocols
Click here to view our Standard Protocols