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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of Jurkat using 22010-1-AP (same clone as 22010-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of mouse testis using 22010-1-AP (same clone as 22010-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PHF20 Polyclonal antibody (22010-1-AP)

    WB analysis of Jurkat using 22010-1-AP (same clone as 22010-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHF20 Polyclonal antibody (22010-1-AP)

    IF Staining of HeLa using 22010-1-AP (same clone as 22010-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22010-1-PBS in a different storage buffer formulation.

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PHF20 Polyclonal antibody, PBS Only

PHF20 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 22010-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22010-1-PBS targets PHF20 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHF20 fusion protein Ag15686 相同性解析による交差性が予測される生物種
Full Name PHD finger protein 20
Calculated molecular weight 1012 aa, 115 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC150178
Gene Symbol PHF20
Gene ID (NCBI) 51230
RRIDAB_2878970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVI0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PHD finger protein 20 (PHF20),also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain.PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20. PHF20 is expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer. PHF20 exists as three alternatively spliced isoforms (150kd,130kd and 120kd) which are encoded by a gene that maps to human chromosome 20 (22072714).