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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CoraLite® Plus 488-conjugated PHOX2B Polyclonal an (CL488-25276)

    IF Staining of SH-SY5Y using CL488-25276

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 488 PHOX2B antibody (CL488-25276) at dilution of 1:600, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 488-conjugated PHOX2B Polyclonal an (CL488-25276)

    FC experiment of Neuro-2a using CL488-25276

    1x10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated PHOX2B Polyclonal antibody (CL488-25276)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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CoraLite® Plus 488-conjugated PHOX2B Polyclonal antibody

PHOX2B Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25276

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

PMX2B, Paired-like homeobox 2B, paired like homeobox 2b, Neuroblastoma Phox, NBPhox

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25276 targets PHOX2B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHOX2B fusion protein Ag13196 相同性解析による交差性が予測される生物種
Full Name paired-like homeobox 2b
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC017199
Gene Symbol PHOX2B
Gene ID (NCBI) 8929
RRIDAB_3672790
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99453
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PHOX2B antibody CL488-25276Download protocol
FC protocol for CL Plus 488 PHOX2B antibody CL488-25276Download protocol
Standard Protocols
Click here to view our Standard Protocols