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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI16 Polyclonal antibody, PBS Only

PI16 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30527-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (30527-1-AP)

    WB analysis using 30527-1-AP (same clone as 30527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PI16 Polyclonal antibody (30527-1-AP)

    WB analysis using 30527-1-AP (same clone as 30527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30527-1-AP (PI16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PI16 Polyclonal antibody (30527-1-AP)

    IF Staining of SKOV-3 using 30527-1-AP (same clone as 30527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PI16 antibody (30527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30527-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30527-1-PBS targets PI16 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI16 fusion protein Ag32556 相同性解析による交差性が予測される生物種
Full Name peptidase inhibitor 16
Calculated molecular weight 463 aa, 49 kDa
Observed molecular weight 100 kDa, 49 kDa
GenBank accession numberBC022399
Gene symbol PI16
Gene ID (NCBI) 221476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.