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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p110 Alpha Polyclonal antibody (27921-1-AP)

    WB analysis using 27921-1-AP (same clone as 27921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p110 Alpha Polyclonal antibody (27921-1-AP)

    WB analysis using 27921-1-AP (same clone as 27921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27921-1-PBS in a different storage buffer formulation.

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PI3 Kinase p110 Alpha Polyclonal antibody, PBS Only

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, Indirect ELISA
Cat No. 27921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27921-1-PBS targets PI3 Kinase p110 Alpha in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PI3 Kinase p110 Alpha fusion protein Ag27539 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight 1068 aa, 124 kDa
Observed molecular weight110 kDa
GenBank accession numberBC113601
Gene Symbol PIK3CA
Gene ID (NCBI) 5290
RRIDAB_2918135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42336
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).