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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PI3 Kinase p110 Alpha Recombinant antibody, PBS Only

PI3 Kinase p110 Alpha Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4

Cat no : 82796-4-PBS

Synonyms

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p110 Alpha Recombinant antibody (82796-4-RR)

    WB analysis using 82796-4-RR (same clone as 82796-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82796-4-RR (PIK3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82796-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82796-4-RR

    Affinity and Kinetic Characterization of 82796-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82796-4-RR against Human PI3 Kinase p110 Alpha were performed. The affinity constant is 0.385 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82796-4-PBS targets PI3 Kinase p110 Alpha in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PI3 Kinase p110 Alpha fusion protein Ag16586 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight 1068 aa, 124 kDa
Observed molecular weight110 kDa
GenBank accession numberBC113601
Gene symbol PIK3CA
Gene ID (NCBI) 5290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).