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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p110 Alpha Recombinant antibody (82796-4-RR)

    WB analysis using 82796-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82796-4-RR (PIK3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82796-4-RR

    Affinity and Kinetic Characterization of 82796-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82796-4-RR against Human PI3 Kinase p110 Alpha were performed. The affinity constant is 0.639 nM.

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PI3 Kinase p110 Alpha Recombinant antibody

PI3 Kinase p110 Alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82796-4-RR
Clone No.1B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82796-4-RR targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PI3 Kinase p110 Alpha fusion protein Ag16586 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight 1068 aa, 124 kDa
Observed molecular weight110 kDa
GenBank accession numberBC113601
Gene Symbol PIK3CA
Gene ID (NCBI) 5290
RRIDAB_3086541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42336
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p110 Alpha antibody 82796-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols