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Target

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Species Reactivity

Species Reactivity

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Conjugate

CoraLite® Plus 488-conjugated PI3 Kinase p85 Beta Monoclonal antibody

PI3 Kinase p85 Beta Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B3B6

Cat no : CL488-67644

Synonyms

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated PI3 Kinase p85 Beta (CL488-67644)

    IF Staining of HeLa using CL488-67644

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PI3 Kinase p85 Beta antibody (CL488-67644, Clone: 2B3B6 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PI3 Kinase p85 Beta (CL488-67644)

    FC experiment of HeLa using CL488-67644

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pi3 Kinase P85 Beta Monoclonal Antibody (CL488-67644, Clone:2B3B6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67644 targets PI3 Kinase p85 Beta in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p85 Beta fusion protein Ag14369 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Calculated molecular weight 728 aa, 82 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC014170
Gene symbol PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PI3 Kinase p85 Beta antibody CL488-67644Download protocol
FC protocol for CL Plus 488 PI3 Kinase p85 Beta antibody CL488-67644Download protocol
Standard Protocols
Click here to view our Standard Protocols