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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated PI3 Kinase p110 Alpha Mono (CL594-67071)

    IF Staining of HeLa using CL594-67071

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PI3 Kinase p110 Alpha antibody (CL594-67071, Clone: 1F6A7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated PI3 Kinase p110 Alpha Mono (CL594-67071)

    FC experiment of Jurkat using CL594-67071

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PI3 Kinase p110 Alpha (CL594-67071, Clone:1F6A7) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®594-conjugated PI3 Kinase p110 Alpha Monoclonal antibody

PI3 Kinase p110 Alpha Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67071
Clone No.1F6A7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration:  100 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥83,000/ 100 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67071 targets PI3 Kinase p110 Alpha in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p110 Alpha fusion protein Ag17392 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight 1068 aa, 124 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC113601
Gene Symbol PIK3CA
Gene ID (NCBI) 5290
RRIDAB_3084745
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42336
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Protocols

Product Specific Protocols
IF protocol for CL594 PI3 Kinase p110 Alpha antibody CL594-67071Download protocol
Standard Protocols
Click here to view our Standard Protocols