Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-82938 targets PI4KA in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | PI4KA fusion protein Ag3062 相同性解析による交差性が予測される生物種 |
| Full Name | phosphatidylinositol 4-kinase, catalytic, alpha |
| Calculated molecular weight | 2044 aa, 231 kDa |
| Observed molecular weight | 231 kDa |
| GenBank accession number | BC018120 |
| Gene Symbol | PI4KA |
| Gene ID (NCBI) | 5297 |
| RRID | AB_3673147 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42356 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PI4KA antibody CL488-82938 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |