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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PIAS2 Recombinant antibody, PBS Only (Capture)

PIAS2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241474G8

Cat no : 84189-4-PBS

Synonyms

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PIAS2 Recombinant antibody (84189-4-RR)

    WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PIAS2 Recombinant antibody (84189-4-RR)

    WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01062-1

    Cytometric bead array standard curve of MP01062-1

    Cytometric bead array standard curve of MP01062-1, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-1-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01062-2

    Cytometric bead array standard curve of MP01062-2

    Cytometric bead array standard curve of MP01062-2, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-2-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84189-4-RR

    Affinity and Kinetic Characterization of 84189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84189-4-PBS targets PIAS2 as part of a matched antibody pair:

MP01062-1: 84189-4-PBS capture and 84189-1-PBS detection (validated in Cytometric bead array)

MP01062-2: 84189-4-PBS capture and 84189-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIAS2 fusion protein Ag9032 相同性解析による交差性が予測される生物種
Full Name protein inhibitor of activated STAT, 2
Calculated molecular weight 63 kDa, 69 kDa
Observed molecular weight63-68 kDa
GenBank accession numberBC015190
Gene symbol PIAS2
Gene ID (NCBI) 9063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.