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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PIAS2 Recombinant antibody (84189-4-RR)

    WB analysis of mouse testis using 84189-4-RR

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PIAS2 Recombinant antibody (84189-4-RR)

    WB analysis using 84189-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84189-4-RR

    Affinity and Kinetic Characterization of 84189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

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PIAS2 Recombinant antibody

PIAS2 Recombinant Antibody for WB, ELISA
Cat No. 84189-4-RR
Clone No.241474G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, mouse testis tissue, HeLa cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84189-4-RR targets PIAS2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PIAS2 fusion protein Ag9032 相同性解析による交差性が予測される生物種
Full Name protein inhibitor of activated STAT, 2
Calculated molecular weight 63 kDa, 69 kDa
Observed molecular weight63-68 kDa
GenBank accession numberBC015190
Gene Symbol PIAS2
Gene ID (NCBI) 9063
RRIDAB_3671742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for PIAS2 antibody 84189-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols