Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, K-562 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 2 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
14413-1-AP targets PIBF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PIBF1 fusion protein Ag5755 相同性解析による交差性が予測される生物種 |
| Full Name | progesterone immunomodulatory binding factor 1 |
| Calculated molecular weight | 90 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC051911 |
| Gene Symbol | PIBF1 |
| Gene ID (NCBI) | 10464 |
| RRID | AB_10858916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXW3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PIBF1 antibody 14413-1-AP | Download protocol |
| IHC protocol for PIBF1 antibody 14413-1-AP | Download protocol |
| IF protocol for PIBF1 antibody 14413-1-AP | Download protocol |
| IP protocol for PIBF1 antibody 14413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
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J Cell Biol A ciliopathy complex builds distal appendages to initiate ciliogenesis.
| ||
EMBO Rep Zika virus alters centrosome organization to suppress the innate immune response. | ||
PLoS Biol The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly
| ||
Cell Rep CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression. | ||
EMBO J Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis |