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  • Featured Product
  • KD/KO Validated

PI3 Kinase p85 Alpha Monoclonal antibody

PI3 Kinase p85 Alpha Monoclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

4G3C11

Cat no : 60225-1-Ig

Synonyms

P85a; PIK3R1, PI3K p85 alpha, PI3K p85 α, PIK3R1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis using 60225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis using 60225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of HEK-293 using 60225-1-Ig

    WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of HEK-293 using 60225-1-Ig

    WB result of PI3 Kinase p85 Alpha antibody (60225-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PI3 Kinase p85 Alpha transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis using 60225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis using 60225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of rat skeletal muscle using 60225-1-Ig

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis using 60225-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of pig brain using 60225-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of Jurkat using 60225-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of A549 using 60225-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of Neuro-2a using 60225-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of PC-12 using 60225-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of rat brain using 60225-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of pig brain using 60225-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of fetal human brain using 60225-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    WB analysis of mouse brain using 60225-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of RAW 264.7 cells using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IP experiment of RAW 264.7 using 60225-1-Ig

    IP result of anti-PI3 Kinase p85 Alpha (IP:60225-1-Ig, 5ug; Detection:60225-1-Ig 1:500) with RAW 264.7 cells lysate 1480ug.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of mouse lung using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of mouse lung using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 60225-1-Ig (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of mouse brain using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of mouse brain using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of human prostate cancer using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of human prostate cancer using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of human skeletal muscle using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PI3 Kinase p85 Alpha Monoclonal antibody (60225-1-Ig)

    IHC staining of human skeletal muscle using 60225-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85(alpha) antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, fetal human brain tissue, Jurkat cells, MCF-10A cells, mouse brain tissue, Neuro-2a cells, PC-12 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, U-251 cells, HT-29 cells, HuT 78 cells, C2C12 cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inmouse lung tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60225-1-Ig targets PI3 Kinase p85 Alpha in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat, pig, canine, bovine, duck
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p85 Alpha fusion protein Ag2344 相同性解析による交差性が予測される生物種
Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC030815
Gene symbol PI3 Kinase p85 Alpha
Gene ID (NCBI) 5295
RRIDAB_11042594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
IHC protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
IP protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomater Res

High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

Authors - Di Sun
View Article
humanWB

Autophagy

The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis.

Authors - Shulan Pi
View Article
humanWB

Oncogene

MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.

Authors - Ran-Ran Ma
View Article
mouseWB

Phytother Res

11-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E2 /ERβ signaling pathways

Authors - Yan Lyu
View Article
ratWB

Environ Pollut

Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats.

Authors - Rundong Liu
View Article
mouseWB

Arterioscler Thromb Vasc Biol

Short-Term Exposure to Waterpipe/Hookah Smoke Triggers a Hyperactive Platelet Activation State and Increases the Risk of Thrombogenesis.

Authors - Ahmed B Alarabi
View Article