Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, fetal human brain tissue, Jurkat cells, MCF-10A cells, mouse brain tissue, Neuro-2a cells, PC-12 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, U-251 cells, HT-29 cells, HuT 78 cells, C2C12 cells |
| Positive IP detected in | RAW 264.7 cells |
| Positive IHC detected in | human placenta tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse brain tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 207 publications below |
| IHC | See 10 publications below |
| IF | See 2 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
60225-1-Ig targets PI3 Kinase p85 Alpha in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, canine, bovine, duck |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | PI3 Kinase p85 Alpha fusion protein Ag2344 相同性解析による交差性が予測される生物種 |
| Full Name | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
| Calculated molecular weight | 85 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC030815 |
| Gene Symbol | PI3 Kinase p85 Alpha |
| Gene ID (NCBI) | 5295 |
| RRID | AB_11042594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P27986 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
| IHC protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
| IP protocol for PI3 Kinase p85 Alpha antibody 60225-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomater Res High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration | ||
Autophagy The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis. | ||
Cell Commun Signal BMAL1 ameliorates type 2 diabetes-induced cognitive impairment via AREG upregulation and PI3K/Akt/GSK-3β pathway activation | ||
Oncogene MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer. | ||
Phytother Res 11-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E2 /ERβ signaling pathways | ||
Environ Pollut Combined exposure of lead and high-fat diet enhanced cognitive decline via interacting with CREB-BDNF signaling in male rats. |