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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated PIP5K1A Polyclonal a (CL488-15713)

    FC experiment of HeLa using CL488-15713

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PIP5K1A (CL488-15713) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PIP5K1A Polyclonal antibody

PIP5K1A Polyclonal Antibody for FC (Intra)
Cat No. CL488-15713

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

PIP5K1-alpha, PIP5K1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, EC:2.7.1.68

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15713 targets PIP5K1A in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIP5K1A fusion protein Ag8340 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
Calculated molecular weight 56 kDa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC007833
Gene Symbol PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2934412
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99755
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 PIP5K1A antibody CL488-15713Download protocol
Standard Protocols
Click here to view our Standard Protocols